[China Instrument Network Instrument R&D] Recently, the National Natural Science Foundation of China's Chemical Science Department held the 2017 “Special Fundamentals for Scientific Instrument Research†project in Beijing. Prof. Tang Bo attended the meeting as the head of the “Special Fluorescence Analyzer for the Detection of Active Small Molecule Species in Single Cells†approved by Shandong Normal University as the first scientific research fund for basic research projects.
This project is based on the research of novel fluorescent probes focusing on active small molecules, and designed key technologies such as multi-functional microfluidics, single-cell analysis chips, dual-laser-induced fluorescence trichromatic detection, and signal acquisition and system control. For the unit module, a fluorescence analyzer suitable for detection of active small molecule species in a single cell was developed; a variety of important small molecules with a large difference in single cell concentration (active oxygen, reactive nitrogen, mercapto compounds, metal ions, etc.) were established. At the same time, a new method for quantitative detection was used to obtain information on the correlation of the content, level changes, and cellular molecular events of these small active molecules within a single cell.
The equipment developed by the project solves the inadequacy of current commercial fluorescence spectrometers for single cell, laser confocal and flow cytometry in accurately quantifying active small molecules in single cells, providing a single cell analysis, small molecule detection and other fields An important scientific instrument. The analytical method established by the project breaks through the bottleneck that it is difficult to acquire quantitative information of many important small molecules in a single cell at the same time. It will provide a new analytical method for studying the physiological and pathological mechanisms associated with active small molecules at the single cell level. During the implementation of the project, 74 SCI papers were published, with a total of 1676 references; 14 applications for invention patents and 5 authorizations; and 2 provincial and ministerial awards for research achievements, and remarkable achievements in personnel training.
The acceptance expert group heard the report of the project leader, reviewed the relevant materials, and watched the video of the normal working status of the instrument core components and instruments. After questioning and commenting, the research project of the project fully completed the research plan and achieved outstanding progress. , passed the acceptance with a comprehensive evaluation of outstanding excellent results.
(Original title: The National Science Instrument Fundamental Research Project undertaken by our school passed the final acceptance test with outstanding results)
This project is based on the research of novel fluorescent probes focusing on active small molecules, and designed key technologies such as multi-functional microfluidics, single-cell analysis chips, dual-laser-induced fluorescence trichromatic detection, and signal acquisition and system control. For the unit module, a fluorescence analyzer suitable for detection of active small molecule species in a single cell was developed; a variety of important small molecules with a large difference in single cell concentration (active oxygen, reactive nitrogen, mercapto compounds, metal ions, etc.) were established. At the same time, a new method for quantitative detection was used to obtain information on the correlation of the content, level changes, and cellular molecular events of these small active molecules within a single cell.
The equipment developed by the project solves the inadequacy of current commercial fluorescence spectrometers for single cell, laser confocal and flow cytometry in accurately quantifying active small molecules in single cells, providing a single cell analysis, small molecule detection and other fields An important scientific instrument. The analytical method established by the project breaks through the bottleneck that it is difficult to acquire quantitative information of many important small molecules in a single cell at the same time. It will provide a new analytical method for studying the physiological and pathological mechanisms associated with active small molecules at the single cell level. During the implementation of the project, 74 SCI papers were published, with a total of 1676 references; 14 applications for invention patents and 5 authorizations; and 2 provincial and ministerial awards for research achievements, and remarkable achievements in personnel training.
The acceptance expert group heard the report of the project leader, reviewed the relevant materials, and watched the video of the normal working status of the instrument core components and instruments. After questioning and commenting, the research project of the project fully completed the research plan and achieved outstanding progress. , passed the acceptance with a comprehensive evaluation of outstanding excellent results.
(Original title: The National Science Instrument Fundamental Research Project undertaken by our school passed the final acceptance test with outstanding results)