The leaves are the main organs of plants for photosynthesis, transpiration, and synthesis of organic matter. They are the basis for the survival of organisms on Earth and the source of energy. The size of the leaves has a significant impact on light energy utilization, water evaporation, dry matter accumulation, yield, and economic benefits. . From a very early time, people began to study how to determine the area of ​​the leaves. There are traditional grid methods, hole weighing methods, and paper weighing methods. However, the grid process is time-consuming and labor-consuming, and the measurement accuracy of irregularly shaped blades is greatly reduced. The perforation weighing method (or direct weighing method) is greatly affected by the blade thickness, leaf age, and leaf water content. Therefore, the error is too large; the paper weight method is said to be affected by the leaf paper and paper texture uniformity of the paper, and it will also produce a certain error; the parabola fitting method or the Simpson formula method is not suitable for leaves with an irregular edge curve; the coefficient method The regression equation method is simple in operation and can be measured in vivo, but a large number of leaves need to be measured in advance to obtain a correction coefficient or an optimal regression model. The error for a single leaf is large, and the specific coefficient or model can only be applied to specific species, varieties, and plants. It is not suitable for varieties with large and irregular leaf shape. The leaf area meter is fast and accurate, but it is expensive and can cause damage to plants. Scanners were used to collect the image of the leaves and use image processing software to determine the length, width, weight, leaf shape index, leaf area index, specific leaf area, and other results. The results were determined using a leaf area detector (high accuracy). Consistent, able to meet most of the needs of the measurement.
We use leaf area analyzer and computer + scanner method to compare: leaf area analyzer method - take a leaf of the plant, clean the upper and lower leaves with dry gauze, press the handle on the cover of the pressure detector, open The leaf area of ​​the living body is covered with a cover, and the leaf is laterally sandwiched to expose the leaf base or petiole. Release the handle and close the upper cover. Hold the probe handle in the right hand and push back the zero button so that the screen shows “0â€. The left hand gently pulls the leaf and measures the length of the wire until the leaf passes completely through the detector and the number displayed on the fluorescent screen is recorded, which is the area of ​​the leaf. The image processing technology was determined by using a scanner to obtain the digital pictures of the plant leaves. The Photoshop software was used to analyze and calculate the leaf area. Open the image of the leaf. Use the “Magnetic Lasso†or “Magic Wand†tool in Photoshop to select the part of the leaf in the image, then open the “Image†in the menu, select the “Histogram†option, and record the “pixel†value in the display parameter. Using the “Lasso†tool in the software, select any known pattern area of ​​the coordinate paper and record the “pixel†value of the selected area as the calculation coefficient. The formula is: leaf area = number of pixels in the blade / number of pixels in the selected square × known pattern area . If the scanned image is scanned according to 100%, no reference is needed. You can directly check the size of the image (preferably in centimeters) and select the entire image to obtain the entire image pixel from the histogram. Leaf Area = Number of Pixels in the Leaf / Whole Image Number of pixels × the entire picture area. The results showed that there was no significant difference between the leaf area measured by the computer + scanner and the results measured with the portable leaf area meter, indicating that it had a high degree of accuracy. In practice, we can comprehensively compare and select the most appropriate measurement method.
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