Heap leaching is a special bacterial microbial growth in an acidic mine water, which many types, such as iron oxide, sulfur bacteria, Thiobacillus ferrooxidans, Thiobacillus type macrophages facultative bacteria resistant, curing some of the genus lobes thermophilic bacteria, poly Thiobacillus and the like. However, the main application in production practice is Thiobacillus ferrooxidans. It has a short rod shape, about 1.0 to 1.5 μm in length and 0.5 μm in width, and exists in a single or in pairs, and can be actively moved, and Gram stain is negative. It is a self-fertilizing bacteria, which uses energy released during the process of oxidizing sulfide ore, elemental sulfur, ferrous sulfate, and arsenic pyrite to synthesize cells and synthesize them in a carbon dioxide source in the air. Its growth and breeding conditions are: the optimum temperature is 25-35 ° C, the growth is slow below 16 ° C, and the death is above 45 ° C; the pH of the medium is 1.5-4.0, and the pH is 1.5-3.0. Must have sufficient oxygen or air and be sensitive to organic matter.
Since Thiobacillus ferrooxidans is an exothermic reaction in the oxidation of sulfide ore, and it cannot adapt to higher temperatures, many scholars actively seek and study heat-resistant strains, such as facultative heat-resistant seedlings S [6] , and vulcanization. The spores, etc., are said to grow and reproduce at 70 °C.
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