Chlorophyll, one of the most widely distributed natural pigments on the earth, is a necessary catalyst for photosynthesis of plants, and is generally present in the leaves of plants and in the stems of green plants. The amount of chlorophyll content is also one of the signs of the health status of a plant. For this reason, the determination of its content can be performed in modern times using a chlorophyll meter. Chlorophyll, as a natural edible pigment, is not only an important additive in foods, but also can be used as a coloring agent in cosmetics and pharmaceutical products and has certain medical applications. The sodium copper chlorophyll can also be used in beverage processing, so the extraction of chlorophyll The study is of great significance. Extraction of chlorophyll from clover should be a good method for extracting a large amount of chlorophyll, which is beneficial to industrial production, and therefore has broad prospects for development.
Take 30g of fresh leaves each time, crush and grind the mixture and add silica and a certain amount of solvent to grind the paste, then add the remaining solvent (calculated according to the ratio of material to liquid) to extract the temperature of 70e, and the ratio of material to liquid is 1B3. The time is 1 h and reflux extraction. After cooling to obtain the chlorophyll extract, the absorbance at 652 nm is measured as the maximum absorption peak and repeated three times to obtain the average absorbance. It can be seen that the extraction solvent ethanol has the highest extraction efficiency, and the extraction effect of acetone and ethanol is not much different. Considering the toxicity of acetone is relatively large and the price is expensive, ethanol is finally determined as the extraction solvent.
The chlorophyll meter was used to determine the content of living organisms. It was found that the extraction time and extraction temperature of chlorophyll were directly proportional to the extraction efficiency of chlorophyll. That is, the longer the extraction time, the higher the extraction temperature was, the higher the extraction efficiency was, but the longer the time, the lower the production. Efficiency, chlorophyll itself is also lost, so the extraction time is not too long, it is necessary to choose an appropriate time, so that it can meet both production and loss to a minimum level. Excessive extraction temperatures can also cause loss of chlorophyll because chlorophyll is easily decomposed by heat. Therefore, in order not to affect the extraction effect and quality, suitable conditions must be found. L9 (34) orthogonal experiments were used for optimal combination to determine the optimal conditions.
Take 30g of fresh leaves each time, crush and grind the mixture and add silica and a certain amount of solvent to grind the paste, then add the remaining solvent (calculated according to the ratio of material to liquid) to extract the temperature of 70e, and the ratio of material to liquid is 1B3. The time is 1 h and reflux extraction. After cooling to obtain the chlorophyll extract, the absorbance at 652 nm is measured as the maximum absorption peak and repeated three times to obtain the average absorbance. It can be seen that the extraction solvent ethanol has the highest extraction efficiency, and the extraction effect of acetone and ethanol is not much different. Considering the toxicity of acetone is relatively large and the price is expensive, ethanol is finally determined as the extraction solvent.
The chlorophyll meter was used to determine the content of living organisms. It was found that the extraction time and extraction temperature of chlorophyll were directly proportional to the extraction efficiency of chlorophyll. That is, the longer the extraction time, the higher the extraction temperature was, the higher the extraction efficiency was, but the longer the time, the lower the production. Efficiency, chlorophyll itself is also lost, so the extraction time is not too long, it is necessary to choose an appropriate time, so that it can meet both production and loss to a minimum level. Excessive extraction temperatures can also cause loss of chlorophyll because chlorophyll is easily decomposed by heat. Therefore, in order not to affect the extraction effect and quality, suitable conditions must be found. L9 (34) orthogonal experiments were used for optimal combination to determine the optimal conditions.
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